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5. images observed using the light microscope are inverted and reversed…

Question

  1. images observed using the light microscope are inverted and reversed. what does this mean?
  2. explain why an image must be centered in the field of view when using low power before moving to a higher power magnification.
  3. describe how the field of view and the amount of available light change when going from high to low power objectives.
  4. explain the difference between magnification and resolving power.
  5. why must you always clean the microscope lenses with lens tissue and lens cleaner, and not with facial tissue, your t - shirt, or any other material?

Explanation:

Response
Question 5
Brief Explanations

When a light microscope inverts and reverses an image, it means the image is flipped both vertically (inverted) and horizontally (reversed). For example, if an object is facing right, its image will face left, and if it's at the top of the specimen, it will appear at the bottom in the view. This is due to the way light passes through the microscope's lenses (objective and ocular), which use convex lenses to form the image, resulting in this orientation change.

Brief Explanations

The field of view (the area visible through the microscope) is larger at low power. When moving to higher power, the field of view becomes smaller. If the image is not centered at low power, when we switch to higher power, the part of the specimen we want to observe may move out of the now - smaller field of view. Also, the stage adjustment range for centering at higher power is very small (since the objective is closer to the specimen), so centering at low power (with a larger field of view and more forgiving stage movement) ensures the object stays in view at higher power.

Brief Explanations
  • Field of View: The field of view is the area that can be seen through the microscope. When going from high - power to low - power objectives, the magnification decreases. A lower magnification means we can see a larger area of the specimen. So the field of view increases (becomes larger) when switching from high to low power.
  • Available Light: The amount of light is related to the size of the objective's aperture (numerical aperture) and the field of view. Low - power objectives usually have a larger field of view and may allow more light to enter (or the light is spread over a larger area, making the overall available light per unit area seem like there is more, or the diaphragm/condenser settings that are set for low power can also affect it). In general, when going from high to low power, the amount of available light increases (the view becomes brighter) because the light is spread over a larger area, and also low - power objectives may have a larger aperture to let in more light.

Answer:

It means the image is flipped vertically (inverted) and horizontally (reversed); e.g., a right - facing object appears left - facing, and a top - positioned object appears at the bottom in the view, due to lens - based image formation.

Question 6