Question

3
1.25 points
ebook
references
inspection
isolation
incubation
identification
inoculation
reset
a guide to
the five is

1. inoculation: sample is placed into a container of growth medium via inoculation. medium can be solid or liquid or a live animal such as a chicken embryo.
2. isolation: an incubator creates the proper growth conditions with respect to temperature and gas requirements.
3. incubation: once the cultures have grown, they may need to be re - inoculated (and incubated) in such a way that separate species are obtained.
4. identification: the colonies on agar or the broth cultures are observed macroscopically and microscopically, possibly with the aid of staining.
5. fifth step (purple box): the identity of the isolated microbe is determined, usually to the species level. inspection may be enough to identify some microbes, but additional techniques include biochemical tests, immunologic tests, and genetic analysis.

petri dish: cdc/james gathany; woman in lab: monty rakusen/getty images; scientist kneeling: pixtal/age fotostock; vibrio cholerae petri dish: centers for disease control and prevention; dna gel electrophoresis: lisa burgess/mcgraw - hill education

Explanation:

To solve this, we analyze each step of the "Five I's" and match with the options:

1. Inoculation: The first step (labeled 1 in the guide) describes placing a sample into growth medium (inoculation). The description for the first step matches "Inoculation" (placing sample into medium).
2. Incubation: The third step (labeled 3) is about growing cultures (incubation: providing growth conditions). Wait, no—wait the labels: Wait the guide’s steps:

• Step 1 (green): Inoculation (sample into medium).
• Step 2 (orange): Isolation? No, step 2’s text: "An incubator creates the proper growth conditions..." Wait, no, the labels in the image: The first step (top green) is "Inoculation" (text: sample into medium). Then step 2 (orange) is "Isolation"? No, the text for step 2: "An incubator creates the proper growth conditions..." Wait, no, the user’s image: Let's re-express:

Looking at the "Five I's" guide:

• 1. Inoculation (green box): "Sample is placed into a container of growth medium via inoculation..."
• 2. Incubation (orange box? Wait no, the numbers: 1 (green), 2 (orange), 3 (blue), 4 (red), 5 (pink). Wait the blue box is labeled 3: "Once the cultures have grown, they may need to be re - inoculated (and incubated) in such a way that separate species are obtained" – no, that’s Isolation? Wait, no, the pink box is labeled 5: "The identity of the isolated microbe is determined..." – that’s Identification? Wait, no, the red box is labeled 4: "The colonies on agar or the broth cultures are observed macroscopically and microscopically..." – that’s Inspection?

Wait, the left - hand options are Inspection, Isolation, Incubation, Identification, Inoculation.

Let's match each option:

• Inoculation: Matches step 1 (green): "Sample is placed into a container of growth medium via inoculation..."
• Incubation: Step 2 (orange) text: "An incubator creates the proper growth conditions..." – so Incubation is about providing growth conditions (incubator).
• Isolation: Step 3 (blue) text: "Once the cultures have grown, they may need to be re - inoculated (and incubated) in such a way that separate species are obtained" – this is Isolation (getting separate species).
• Inspection: Step 4 (red) text: "The colonies on agar or the broth cultures are observed macroscopically and microscopically..." – this is Inspection (observing colonies).
• Identification: Step 5 (pink) text: "The identity of the isolated microbe is determined..." – this is Identification (determining microbe identity).

If we assume the question is to match the first step (green box) with the option, the answer is Inoculation. If it's another step, we adjust. But based on the first step’s description, the answer is Inoculation.

Answer:

Inoculation