Question

drag the descriptions to the correct type of medium to test your understanding of various selective and differential media. acidic media which allows for the growth of fungi; neutral red allows for identification of lactose fermenting bacteria; malachite green allows for the growth of mycobacterium; bile and crystal violet allow for the growth of gram - negative enterics; sodium azide allows for the growth of fecal enterococci; identification of hydrogen sulfide producing bacteria; phenol red allows for identification of staphylococcal species; demonstrates hemolysis of streptococcus species. selective media: enterococcus faecalis broth, macconkey agar, lowenstein - jensen, sabouraud’s agar. differential media: blood agar, mannitol salt agar, triple - sugar iron agar, macconkey agar.

Explanation:

Selective Media:

• Enterococcus faecalis broth: Sodium azide allows for the growth of fecal enterococci
• MacConkey Agar: Bile and crystal violet allow for the growth of gram - negative enterics
• Lowenstein - Jensen: Malachite green allows for the growth of Mycobacterium
• Sabouraud’s Agar: Acidic media which allows for the growth of fungi

Differential Media:

• Blood Agar: Demonstrates hemolysis of Streptococcus species
• Mannitol Salt Agar: Phenol red allows for identification of Staphylococcal species
• Triple - sugar Iron Agar: Identification of hydrogen sulfide producing bacteria
• MacConkey Agar: Neutral red allows for identification of lactose fermenting bacteria

for Each Match:

• Selective Media Principle: Selective media contain components that inhibit the growth of some microbes and promote the growth of others. For example, in Enterococcus faecalis broth, sodium azide inhibits most bacteria except fecal enterococci. MacConkey Agar uses bile and crystal violet to inhibit gram - positive bacteria, favoring gram - negative enterics. Lowenstein - Jensen uses malachite green to inhibit other bacteria and support Mycobacterium growth. Sabouraud’s Agar’s acidic environment inhibits many bacteria and supports fungal growth.
• Differential Media Principle: Differential media allow different microbes to grow but show visible differences (like color change, hemolysis) that help identify them. Blood Agar shows hemolysis patterns of Streptococcus. Mannitol Salt Agar uses phenol red to detect Staphylococcal mannitol fermentation. Triple - sugar Iron Agar detects hydrogen sulfide production. MacConkey Agar uses neutral red to detect lactose fermentation by bacteria.

Answer:

for Each Match:

• Selective Media Principle: Selective media contain components that inhibit the growth of some microbes and promote the growth of others. For example, in Enterococcus faecalis broth, sodium azide inhibits most bacteria except fecal enterococci. MacConkey Agar uses bile and crystal violet to inhibit gram - positive bacteria, favoring gram - negative enterics. Lowenstein - Jensen uses malachite green to inhibit other bacteria and support Mycobacterium growth. Sabouraud’s Agar’s acidic environment inhibits many bacteria and supports fungal growth.
• Differential Media Principle: Differential media allow different microbes to grow but show visible differences (like color change, hemolysis) that help identify them. Blood Agar shows hemolysis patterns of Streptococcus. Mannitol Salt Agar uses phenol red to detect Staphylococcal mannitol fermentation. Triple - sugar Iron Agar detects hydrogen sulfide production. MacConkey Agar uses neutral red to detect lactose fermentation by bacteria.