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Question
great job! what values do we need to calculate first, before we can plot the michaelis-menten curve?
a) initial reaction rate
b) $v_{max}$
c) maximum absorbance
d) $k_m$
The Michaelis-Menten curve plots initial reaction rate ($V_0$) on the y-axis against substrate concentration ($[S]$) on the x-axis. $V_{max}$ and $K_m$ are derived from the curve, not prerequisites for plotting it. Maximum absorbance is not directly used for the core curve plot. Initial reaction rates must first be calculated at different substrate concentrations to generate the curve.
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a) Initial reaction rate