Question

guiding questions

1. use the plasmid dna below and identify the t7 promoter, origin of replication, laci repressor
2. what is the name of the plasmid?
3. what is the size of the plasmid dna?
4. identify 3 types of restriction enzymes:

Explanation:

Step1: Identify elements on plasmid

Locate labels on the circular plasmid diagram.

Step2: Determine plasmid name

Read the name directly from the diagram.

Step3: Find plasmid size

Read the size value next to the plasmid name.

Step4: List restriction enzymes

Identify enzyme names marked on the plasmid circle.

Answer:

1. To identify the T7 promoter, Origin of replication, and LacI repressor on the plasmid DNA, one would need to look for the specific labeled regions on the circular diagram. The T7 promoter is usually labeled as "T7 promoter" near the start - site of transcription. The origin of replication is often marked as "ori" or has a specific sequence region dedicated to replication initiation. The LacI repressor - coding region is typically labeled as "LacI - coding sequence".
2. The name of the plasmid is pET - 22b(+).
3. The size of the plasmid DNA is 5450bp as indicated on the diagram next to the plasmid name.
4. Three types of restriction enzymes visible on the diagram are BamHI, EcoRI, and HindIII.