Question

lets find out how much you learned about this topic! this will help us personalize your learning experience, and dont worry, it wont affect your grades. question 8 of 11 fluorescence microscopy relies on the use of specific molecules called fluorophores (or fluorescent dyes). these molecules function by: absorbing all wavelengths of light, making the sample appear dark. reacting chemically with cellular components to produce a permanent color change visible under white light. absorbing light energy at a specific excitation wavelength and then emitting light at a longer wavelength, which is then detected.

Explanation:

Fluorophores in fluorescence microscopy absorb light at a specific excitation wavelength and then emit light at a longer wavelength. This emitted light is detected to visualize the sample. The first option is incorrect as they don't absorb all wavelengths to make the sample dark. The second option is wrong because they don't react chemically to produce a permanent color - change visible under white light.

Answer:

Absorbing light energy at a specific excitation wavelength and then emitting light at a longer wavelength, which is then detected.