Question

matching question
match negative staining and shadowing in tem with the correct description.
instructions
negative staining
shadowing
specimen is coated with a thin film of platinum or other heavy metal so that only one side is coated with metal.
specimen is spread out in a thin film with with either phosphotungstic acid or uranyl acetate.
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Explanation:

Negative staining uses heavy metal acids (like phosphotungstic acid, uranyl acetate) to surround the specimen, contrasting the sample against a dark background. Shadowing coats one side of the specimen with a heavy metal (like platinum) to create a 3D-like contrast in TEM by casting a shadow of the sample.

Answer:

Negative staining → Specimen is spread out in a thin film with either phosphotungstic acid or uranyl acetate.
Shadowing → Specimen is coated with a thin film of platinum or other heavy metal so that only one side is coated with metal.