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match negative staining and shadowing in tem with the types of specimens that are visualized.
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negative staining
shadowing
used to visualize viruses and cellular microbes
used to study bacterial flagella and dna
Negative staining in TEM uses an acidic dye that does not penetrate the specimen, creating a dark background around delicate structures like bacterial flagella and DNA, making them visible. Shadowing involves coating the specimen with a heavy metal at an angle, which creates a shadow effect ideal for visualizing the surface structure of larger specimens like viruses and cellular microbes.
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Negative staining → Used to study bacterial flagella and DNA
Shadowing → Used to visualize viruses and cellular microbes