Question

name: date: 7/50/23 period: use a well - plate to analyze complex sugars and proteins: biuret bradford iodine biuret use a disposable pipette and do the analysis of the control and patients using a test strip for the urinalysis. focus questions: 1. what is the reagent used to identify simple sugars or reducing sugars? a. biuret b. benedicts c. iodine d. sudan 2. what is the reagent to identify complex carbohydrates? a. biuret b. benedicts c. iodine d. sudan 3. how are proteins used in our body? list 5 functions. 4. a positive benedicts test results in a color change from blue to: a. purple b. brick - red precipitate c. dark blue d. milky white 5. the biuret test is used to detect: a. lipids b. starch c. proteins d. glucose 6. what is the color change when starch is present with iodine? a. blue to red b. yellow to orange c. brown to blue - black d. colorless to red 7. a positive results using the biurets assay, it changes from __________ to ________ color. a positive results using the bradfords assay, it changes from ________ to __________ color. 8. based on the results of biochemistry tests in urinalysis (benedicts test, biuret test, and the use of the strip tests), what can be concluded about the presence of macromolecules in urine, and how might these results indicate potential health conditions?

Explanation:

1. Benedict's reagent reacts with reducing sugars to form a colored precipitate.
2. Iodine forms a blue - black complex with starch, a complex carbohydrate.
3. Proteins have multiple essential functions in the body as described.
4. Benedict's test with reducing sugars gives a brick - red precipitate in positive cases.
5. The Biuret test is specific for proteins.
6. Iodine reacts with starch to give a blue - black color.
7. Biuret and Bradford assays have characteristic color changes for positive results.
8. Biochemical tests in urinalysis are used to detect macromolecules which can be related to different health conditions based on their normal absence or presence levels in urine.

Answer:

1. B. Benedict's
2. C. iodine
3. - Structural support (e.g., collagen in connective tissues).

• Enzyme - catalyze biochemical reactions.
• Transport (e.g., hemoglobin for oxygen transport).
• Immune response - antibodies are proteins.
• Hormonal regulation (e.g., insulin).

1. B. Brick - red precipitate
2. C. Proteins
3. C. Brown to blue - black
4. A positive Biuret's assay changes from blue to purple; a positive Bradford's assay changes from brown to blue.
5. Benedict's test can detect reducing sugars (such as glucose) in urine. A positive result may indicate diabetes or other metabolic disorders. The Biuret test can detect proteins in urine. Proteinuria (presence of protein in urine) may indicate kidney damage or other kidney - related issues. Strip tests can detect multiple substances like glucose, protein, etc. Overall, the presence of macromolecules in urine can be an indicator of various health problems related to metabolism or organ function.