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Question
question 10
simple staining is often necessary to improve contrast in which microscope?
a phase - contrast microscope
b fluorescence microscope
c electron microscope
d darkfield microscope
e compound light microscope
Phase - contrast microscopes enhance contrast by exploiting differences in refractive index without staining. Fluorescence microscopes use fluorescent dyes for contrast. Electron microscopes use electrons instead of light and have their own sample - preparation methods. Darkfield microscopes create contrast by illuminating specimens against a dark background. Compound light microscopes often require simple staining to improve contrast as the specimens are often transparent or have low natural contrast.
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E. compound light microscope