Question

scientists have compared the dna of stickleback with and without pelvic spines to better understand the dna sequences involved. the gene that controls the development of a pelvic spine is called pitx1. the model below shows a region of dna including pitx1 and a non - coding regulatory region near pitx1 that scientists have found to be related to pelvic spine development.
model of stickleback dna section
which three questions should the scientists investigate in order to better understand the reason that some stickleback fish have pelvic spines and some do not?
○ how does the non - coding regulatory region affect pitx1?
○ are there differences between the pitx1 gene in fish with and without pelvic spines?
○ how did the scientists figure out that pitx1 is related to the development of pelvic spines in stickleback fish?
○ are there differences between the non - coding regulatory region in fish with and

Explanation:

The goal is to identify the question that does not help explain why some sticklebacks have pelvic spines and others do not.

• Questions about the regulatory region's effect on Pitx1, differences in the Pitx1 gene, and differences in the non-coding region all directly address the biological mechanism behind pelvic spine development.
• The question about how scientists discovered the link between Pitx1 and pelvic spines focuses on the research process, not the biological reason for the trait difference itself.

Answer:

How did the scientists figure out that Pitx1 is related to the development of pelvic spines in stickleback fish?