QUESTION IMAGE
Question
what is the function of a spectrophotometer?
click the view theory button to get more information about the spectrophotometer.
a) measure the weight of the cuvette
b) measure the amount of light that passes through the cuvette
c) measure the molecular mass of the enzyme
d) measure the wavelength that passes through the cuvette
A spectrophotometer operates by passing light of a specific wavelength through a sample in a cuvette, then quantifying the amount of transmitted light to determine sample properties like concentration. Option a is incorrect as weight measurement is for balances. Option c is incorrect as molecular mass is measured via techniques like mass spectrometry. Option d is incorrect because the device uses a fixed/selected wavelength source, it does not measure the passing wavelength.
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b) Measure the amount of light that passes through the cuvette