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Question
which of the following is the correct method to maintain aseptic technique in a vertical flow hood per usp <797> guidelines? placing hands directly over the vial or ampule stocking the hood with all supplies needed for the day turning the hood on 15 min prior to cleaning using a filter needle when pulling a solution from an ampule
Placing hands directly over vial/ampule can contaminate, stocking hood with daily supplies can introduce dust and clutter, and turning hood on 15 min prior to use is not the key aseptic - technique. Using a filter needle when drawing from an ampule helps prevent particulate matter and maintain sterility as per USP <797> guidelines.
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Using a filter needle when pulling a solution from an ampule