Question

why is the microscopy slide shining with a blue light? you can see the image by clicking the view image button. a) the blue light has a longer wavelength than the green light; therefore, it excites the fluorophore b) phalloidin is excited by blue light analogous to dapi c) the green fluorescent dye attached to phalloidin is excited by blue light and emits green light d) the high energy blue light initiates the cross - linking of actin and phalloidin

Explanation:

Blue light can excite certain fluorescent dyes. Phalloidin can be attached to a green - fluorescent dye which gets excited by blue light and emits green light. Option a is incorrect as blue light has a shorter wavelength than green light. Phalloidin is not excited by blue light in the same way as DAPI (option b is wrong). Blue light does not initiate cross - linking of actin and phalloidin (option d is wrong).

Answer:

C. The green fluorescent dye attached to phalloidin is excited by blue light and emits green light