Question

in this activity, you will identify which items involve aseptic technique. pasteur’s experiments and observations provided the basis for developing aseptic techniques that prevent the introduction of unwanted microorganisms into controlled environments. given what you have learned from the design of pasteur’s experiment, which of the following scenarios would be considered an aseptic technique? select all statements that apply. view available hint(s) □ allowing sterile solutions to be openly exposed to the air □ blowing on heated, sterile surfaces with your mouth in order to facilitate cooling □ using a bunsen burner (flame) to heat a wire inoculating loop prior to use □ wearing gloves when handling sterile laboratory equipment □ taking steps to prevent contaminants from entering sterile solutions submit next provide feedback

Explanation:

• Allowing sterile solutions to be openly exposed to air would introduce contaminants, so this is not an aseptic technique.
• Blowing on heated, sterile surfaces with the mouth introduces contaminants from saliva and breath, so this is not an aseptic technique.
• Using a Bunsen burner to heat an inoculating loop prior to use sterilizes the loop, preventing contamination of the culture, so this is an aseptic technique.
• Wearing gloves when handling sterile laboratory equipment prevents contaminants from hands (like skin microbes, oils) from entering the sterile equipment, so this is an aseptic technique.
• Taking steps to prevent contaminants from entering sterile solutions is a core part of aseptic technique, as it maintains the sterility of the solution.

Answer:

• using a Bunsen burner (flame) to heat a wire inoculating loop prior to use
• wearing gloves when handling sterile laboratory equipment
• taking steps to prevent contaminants from entering sterile solutions