Question

given the standard curve below, what is the concentration of a sample with * 1 point an absorbance of 0.2? absorbance, 595 nm protein concentration, mg/l 210 mg/l 650 mg/l 2000 mg/l 1474 mg/l

Explanation:

Step1: Locate absorbance on y - axis

Find 0.2 on the absorbance (y - axis).

Step2: Read corresponding x - value

Draw a horizontal line from 0.2 on the y - axis to the standard curve, then draw a vertical line down to the x - axis. The x - axis represents protein concentration. Reading from the graph, the protein concentration corresponding to an absorbance of 0.2 is approximately 210 mg/l.

Answer:

210 mg/l