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1 how would you grow a streptomycin - resistant strain e. coli bacteria was spread on both agar plates and incubated for 24 hours. if you wanted to grow up a large quantity of streptomycin resistant e. coli, what would you do next?
To grow a large quantity of streptomycin - resistant E. coli, pick the resistant colonies from the streptomycin - containing agar plate and transfer them to a liquid medium (such as LB broth) containing streptomycin. The liquid medium provides more nutrients and space for bacterial growth compared to the solid agar plate. The streptomycin in the liquid medium will ensure that only the resistant bacteria survive and multiply.
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Pick the resistant colonies from the streptomycin - containing agar plate and transfer them to a liquid medium (e.g., LB broth) containing streptomycin.