Question

microscope lab sheet
name: teclana velasquez period 2 date: sep 15
part a: label the microscope
below is a diagram of a compound light microscope. fill in the blanks with the correct part names.
microscope labeling
microscope use:

1. when focusing a specimen, you should always start with the ______________ objective.
2. when using the high power objective, only the ______________ knob should be used.
3. the type of microscope used in most science classes is the ______________ microscope.
4. you should carry the microscope by the ____________ and the ____________.
5. the objectives are attached to what part of the microscope (it can be rotated to click lenses into place?) ______________
6. a microscope has an ocular objective of 10x and a high - power objective of 50x. what is the microscopes total magnification? ______________

part b: the “letter e” activity

1. place the slide of the printed letter “e” under the microscope at low power.
2. focus carefully and observe the orientation.
3. question: how does the “e” appear when viewed under the microscope compared to the slide?

answer: ______________

1. part c: sketch what you see

low power (40x total magnification)

1. high power (400x total magnification)

24 - 25. part d: reflection
question: why is it important to start focusing with low power before moving to high power?
answer: ______________

Explanation:

1. Low - power objectives have a larger field of view and are easier to focus on the specimen initially.
2. The fine adjustment knob is used with high - power objectives to make small, precise focus adjustments without crashing the lens into the slide.
3. Compound light microscopes are commonly used in science classes due to their ability to magnify specimens up to 1000x and their relatively simple operation.
4. Carrying a microscope by the arm and base provides stability and helps prevent damage.
5. The objectives are attached to the nosepiece, which can be rotated to change the magnification.
6. Total magnification is calculated by multiplying the magnification of the ocular lens by the magnification of the objective lens. So, \(10\times50 = 500x\).
7. Under a microscope, the image is inverted and reversed, so the "e" will appear upside - down and backward compared to the slide.

24 - 25. Starting with low power allows you to locate the specimen easily as it has a wider field of view. It also reduces the risk of damaging the slide or the lens since the working distance is larger at low power. Once the specimen is located and roughly focused, high - power can be used for more detailed examination.

Answer:

1. low - power
2. fine adjustment
3. compound light
4. arm; base
5. nosepiece
6. 500x
7. Upside - down and backward

24 - 25. It allows easy specimen location due to a wider field of view and reduces the risk of damage as the working distance is larger at low power.