Question

multiple choice question
in negative staining of tem specimens, the ______ appears dark.

• cell
• cell membrane
• background
• nucleus

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Explanation:

In negative staining for TEM, the stain accumulates around the specimen (cells, their membranes, or nuclei) rather than staining the specimen itself. The electron-dense stain in the background absorbs more electrons, making it appear dark, while the unstained specimen appears light against this dark background.

Answer:

C. background