Question

test tube,environmental ph,starch added,amylase added,iodine added,pretreated by boiling
i,5,yes,yes,yes,no
ii,6,yes,yes,yes,no
iii,7,no,yes,yes,no
iv,7,yes,no,yes,no
v,7,yes,yes,yes,yes
vi,7,yes,yes,yes,no
vii,8,yes,yes,yes,no
viii,9,yes,yes,yes,no
which of the following statements best justifies the inclusion of test tube v as a control in the experiment?
a it will provide a measurement of amylase activity at an acidic ph.
b it will provide a measurement of amylase activity at a basic ph.
c it will show the color change that occurs in the absence of enzyme activity.
d it will show the color change that occurs in the absence of the amylase protein.

Explanation:

In the experiment, test - tube V has been pretreated by boiling which denatures the amylase enzyme. This tube has starch, amylase (denatured by boiling), iodine and is at pH 7. By comparing it with other non - boiled tubes with active amylase, it will show the color change that occurs in the absence of enzyme activity. The color change is due to the reaction between iodine and starch, and when amylase is active it breaks down starch, altering the color change.

Answer:

C. It will show the color change that occurs in the absence of enzyme activity.