Question

use the questions below to see how well you understand the methods by which geneticists identify and copy genes of interest.
which of the following can be used to make copies of a gene of interest?
choose all that apply
a restriction enzymes
b dna cloning
c polymerase chain reaction
d dna sequencing

Explanation:

• Restriction enzymes cut DNA at specific sites but do not copy genes.
• DNA cloning inserts a gene into a vector (like a plasmid) to generate multiple copies via host cell replication.
• Polymerase chain reaction (PCR) is an in vitro technique that rapidly amplifies (copies) specific gene segments.
• DNA sequencing determines the order of nucleotides in a gene, it does not copy genes.

Answer:

b. DNA cloning
c. Polymerase chain reaction