Question

use the scenario to answer the question.
a scientist studies the genome of a bacterium that will be genetically engineered to produce human insulin. the nucleotide sequence of the bacterial plasmid dna is decoded. the image shows a segment of the bacterial plasmid dna into which the gene for human insulin will be inserted.
5 agt agc acc gaa ttc cgc gtt 3
3 tca tcg tgg ctt aag gcg caa 5
what is the mrna that will be coded from the 5 to 3 original plasmid dna?
○ acu acg agc caa uug gcg cuu
○ uca ucg ugg cuu aag gcg caa
○ tca tcg tgg ctt aag gcg caa
○ ugu ugc ucc guu ttc cgc gtt

Explanation:

Step1: Identify template DNA strand

The mRNA is synthesized using the 3' to 5' DNA strand as a template. The given 3' to 5' template strand is:
3' TCA TCG TGG CTT AAG GCG CAA 5'

Step2: Apply base pairing rules

In transcription, DNA base pairs with mRNA as follows: T→A, C→G, G→C, A→U. Match each base in the template strand to its mRNA complement, and write mRNA 5' to 3':

• T→A, C→G, A→U; T→A, C→G, G→C; T→A, G→C, G→C; C→G, T→A, T→A; A→U, A→U, G→C; G→C, C→G, G→C; C→G, A→U, A→U

Resulting mRNA sequence (5' to 3'):
5' AGU AGC ACC GAA UUC CGC GUU 3'

Step3: Match to options

Compare the generated sequence to the given options.

Answer:

A. AGU AGC ACC GAA UUC CGC GUU