Question

which statement is true?
a) the coat proteins of the virus have fluorescent properties and appear red if they are excited with uv light
b) actin fibers will be green, because the dye linked to phalloidin absorbs green light
c) the filter cubes consist of an excitation filter, dichroic mirror and emission filter
d) dapi is a blue fluorescent protein linked to an antibody that specifically binds dna

Explanation:

a) Coat proteins of viruses don't inherently have fluorescent properties to appear red on UV - excitation.
b) Actin - phalloidin dyes emit green light, not absorb it to appear green.
c) Filter cubes in fluorescence microscopy indeed consist of an excitation filter, dichroic mirror, and emission filter.
d) DAPI is a blue - fluorescent dye, not a protein linked to an antibody.

Answer:

C. The filter cubes consist of an excitation filter, dichroic mirror and emission filter