Question

if you wanted to purify the insulin receptor from a mixture of proteins, what methods could you use? choose two of the following selections. choose one or more: a. use immunoprecipitation using insulin as a ligand. b. use affinity chromatography with beads coated with insulin. c. use affinity chromatography with beads coated with insulin receptor. d. use affinity chromatography with beads coated with anti - insulin receptor antibodies.

Explanation:

Affinity chromatography with beads coated with insulin can bind to the insulin - receptor due to the specific interaction between insulin and its receptor. Also, affinity chromatography with beads coated with anti - insulin receptor antibodies can specifically bind to the insulin receptor as antibodies have high specificity for their antigen (the insulin receptor in this case). Immunoprecipitation using insulin as a ligand is not a standard approach for this purpose and coating beads with the insulin receptor itself is not a method to purify the insulin receptor from a protein mixture.

Answer:

B. Use affinity chromatography with beads coated with insulin.
D. Use affinity chromatography with beads coated with anti - insulin receptor antibodies.