Question

1. once a specific sample of dna has been isolated how does the cycle sequencing process work? (5pts) in your answer include the words: primer, dna fragments, pathogen dna, dna polymerase, free nucleotides, fluorescently tagged free nucleotides, automatic sequencer, laser, detector, computer.

Explanation:

The process starts with a primer binding to the DNA sample. Polymerase then uses free nucleotides, including fluorescently - tagged ones, to build complementary DNA fragments. As the fragments are formed, a laser excites the fluorescent tags. A detector picks up the light signals from the tags, and these signals are sent to a computer. The computer analyzes the signals to determine the sequence of nucleotides in the DNA sample. This process can be used to analyze pathogen DNA or other DNA samples with an automatic sequencer.

Answer:

The primer binds to the isolated DNA sample. Polymerase uses free nucleotides, including fluorescently - tagged free nucleotides, to create DNA fragments. A laser excites the fluorescent tags on the nucleotides in the fragments. A detector senses the light emitted by the tags and sends the information to a computer. The computer interprets the data to determine the DNA sequence. This process can be carried out using an automatic sequencer for analyzing pathogen DNA or other DNA samples.