Question

why do we need to add buffer into the master mix?
a) to stabilize reaction ph
b) to remove color from the master mix
c) to stabilize master mix temperature
d) to increase master mix volume

Explanation:

Buffers are solutions that resist changes in pH. In biological/chemical reaction master mixes (like in PCR or lab assays), maintaining a stable pH is critical for enzyme function and reaction consistency. The other options are incorrect: buffers do not remove color, stabilize temperature, or are added to increase volume.

Answer:

a) To stabilize reaction pH