Question

1. label the correct tube dilution and final dilution for each test tube below. 1x stock tube dilution final dilution tube dilution final dilution tube dilution final dilution tube dilution final dilution tube dilution final dilution

Explanation:

Step1: Calculate first - tube dilution

The first tube has 1 mL of stock added to 5 mL of diluent. The tube dilution is $\frac{1}{1 + 5}=\frac{1}{6}$. The final dilution is also $\frac{1}{6}$ since it's the first step.

Step2: Calculate second - tube dilution

1 mL of the first - tube solution is added to 5 mL of diluent. The tube dilution is $\frac{1}{1+5}=\frac{1}{6}$. The final dilution is $\frac{1}{6}\times\frac{1}{6}=\frac{1}{36}$.

Step3: Calculate third - tube dilution

2 mL of the second - tube solution is added to 4 mL of diluent. The tube dilution is $\frac{2}{2 + 4}=\frac{1}{3}$. The final dilution is $\frac{1}{36}\times\frac{1}{3}=\frac{1}{108}$.

Step4: Calculate fourth - tube dilution

3 mL of the third - tube solution is added to 3 mL of diluent. The tube dilution is $\frac{3}{3+3}=\frac{1}{2}$. The final dilution is $\frac{1}{108}\times\frac{1}{2}=\frac{1}{216}$.

Step5: Calculate fifth - tube dilution

1 mL of the fourth - tube solution is added to 5 mL of diluent. The tube dilution is $\frac{1}{1+5}=\frac{1}{6}$. The final dilution is $\frac{1}{216}\times\frac{1}{6}=\frac{1}{1296}$.

Answer:

First tube: Tube Dilution = $\frac{1}{6}$, Final Dilution = $\frac{1}{6}$
Second tube: Tube Dilution = $\frac{1}{6}$, Final Dilution = $\frac{1}{36}$
Third tube: Tube Dilution = $\frac{1}{3}$, Final Dilution = $\frac{1}{108}$
Fourth tube: Tube Dilution = $\frac{1}{2}$, Final Dilution = $\frac{1}{216}$
Fifth tube: Tube Dilution = $\frac{1}{6}$, Final Dilution = $\frac{1}{1296}$