Question

1. describe how an indirect elisa test works for meningitis. you must in include the following in your explanation: micro wells, meningitis antigens, primary antibody, secondary antibody, wash, enzyme, substrate, positive and negative results.

Explanation:

1. Coating: Meningitis antigens are adsorbed onto the surface of micro - wells.
2. Primary Antibody Binding: Patient's serum containing primary antibodies (if present) is added. If the patient has been exposed to meningitis, primary antibodies will bind to the antigens.
3. Wash: Unbound substances, including unbound primary antibodies, are washed away.
4. Secondary Antibody Binding: A secondary antibody, which is enzyme - conjugated, is added. It binds to the primary antibody.
5. Wash: Again, unbound secondary antibodies are removed.
6. Substrate Addition: A substrate for the enzyme is added. If the secondary antibody - enzyme complex is present (indicating a positive result), the enzyme will act on the substrate to produce a color change.
7. Result Interpretation: A color change (positive result) indicates the presence of antibodies against meningitis in the patient's serum. No color change (negative result) means no such antibodies were detected.

Answer:

The indirect ELISA test for Meningitis works as described above with the key steps involving antigen coating, antibody binding, washing, and substrate - enzyme reaction for result determination.