Question

consider a situation in which the enzyme is operating at optimum temperature and ph, and has been saturated with substrate. what is your best option for increasing the rate of the reaction?
increase the ph.
increase the temperature.
increase the enzyme concentration.
increase the substrate concentration.

Explanation:

When an enzyme is operating at optimum temperature and pH and is saturated with substrate, the active - sites of the enzyme are already occupied by substrate molecules. Increasing pH or temperature from the optimum will denature the enzyme and decrease the reaction rate. Increasing substrate concentration will not have an effect as the enzyme is already saturated. Increasing enzyme concentration will provide more active - sites for the substrate to bind to, thus increasing the reaction rate.

Answer:

Increase the enzyme concentration.