Question

culture transfer techniques (continued)
the video within the link below shows a type of streak plate technique.
http://www.sumanasinc.com/webcontent/animations/content/streakplate.html
a written version of the streak plate technique or 4 - quadrant streak is found below.
procedure

1. sterilize the inoculating loop in the bunsen burner by clicking on the loop and dragging it to the burner. put the loop into the flame until it is red hot. allow it to cool.
2. pick an isolated colony from the agar plate culture and spread it over the first quadrant (approximately 1/4 of the plate) using close parallel streaks.
3. flame the loop.
4. turn the plate 90° and lightly sweep the loop 1 - 2 times through the inoculated area, then streak into the next quadrant without overlapping the previous

streaks.

1. flame the loop.
2. turn the plate 90°, overlap the previous area 1 - 2 times, and streak into the next quadrant as in step 4.
3. flame the loop.
4. repeat #6, streaking the remainder of the plate.
5. invert the plate and incubate.

how many times do you need to take microorganisms from the stock culture in order to perform a streak plate?
○ 3
○ 4
○ 1
○ 2

Explanation:

To determine how many times microorganisms are taken from the stock culture for a streak plate, we analyze the procedure: Step 2 says to pick an isolated colony (take microorganisms) from the agar plate culture (stock - like source) and spread it over the first quadrant. Subsequent steps (4, 6, 8) involve streaking from the previously inoculated area (not taking from the stock again). So only 1 time.

Answer:

C. 1 (assuming the options are labeled as A. 3, B. 4, C. 1, D. 2)