Question

1. determine the volume in ml to prepare a 1x tgs buffer in 40 ml of di water using a stock solution of 10x tgs buffer. report your answer to one decimal point.

Explanation:

Step1: Use dilution formula

$C_1V_1 = C_2V_2$
where $C_1$ is the concentration of the stock solution, $V_1$ is the volume of the stock solution, $C_2$ is the final concentration, and $V_2$ is the final volume.
Here, $C_1 = 10X$, $C_2=1X$, and $V_2 = 40$ mL.

Step2: Solve for $V_1$

$V_1=\frac{C_2V_2}{C_1}=\frac{1X\times40\text{ mL}}{10X}= 4.0$ mL

Answer:

4.0