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8. dilutions: find the volume to prepare a 1x tae buffer in 500 ml of d…

Question

  1. dilutions: find the volume to prepare a 1x tae buffer in 500 ml of di water using a stock solution of 50x tae buffer. report your answer to one decimal point.

Explanation:

Step1: Use dilution formula

$C_1V_1 = C_2V_2$
where $C_1$ is the concentration of the stock solution, $V_1$ is the volume of the stock solution, $C_2$ is the concentration of the final solution, and $V_2$ is the volume of the final solution.

Step2: Identify given values

$C_1 = 50X$, $C_2=1X$, $V_2 = 500$ mL

Step3: Rearrange formula for $V_1$

$V_1=\frac{C_2V_2}{C_1}$

Step4: Substitute values

$V_1=\frac{1X\times500\text{ mL}}{50X}$

Step5: Calculate result

$V_1 = 10.0$ mL

Answer:

10.0 mL