QUESTION IMAGE
Question
directions: below is a snapshot of dna replication. use the cards below to correctly label the diagram.
cards for labeling:
leading strand
okazaki fragment
dna polymerase
helicase
dna ligase
dna polymerase
cards for labeling (right side):
dna primase
lagging strand
ssb protein
original strand
topoisomerase
rna primer
To label the DNA replication diagram, we analyze each component:
- Helicase: Unwinds the double - helix (the green structure is Helicase, already labeled, but for other parts:
- The blue oval - shaped structure holding the original double - helix is related to the start, but the “Original Strand” is the parental DNA.
- The yellow - arrowed Helicase unwinds, so the area it’s acting on has the Original Strand (the double - helix before unwinding).
- The single - stranded regions after unwinding are stabilized by SSB Protein (Single - Stranded Binding Protein).
- DNA Primase lays down RNA primers (the small segments for DNA Polymerase to start).
- The Leading Strand is synthesized continuously (the long, unbroken new strand), and the Lagging Strand is synthesized in fragments (Okazaki Fragments), which are later joined by DNA Ligase. The Lagging Strand is the one with Okazaki Fragments.
Key Labeling (matching to the dashed boxes and structures):
- The dashed box near the start (double - helix) → Original Strand (parental DNA).
- The single - stranded regions (after Helicase) → SSB Protein (stabilizes single strands).
- The small primer - like structures → RNA Primer (laid by Primase).
- The long, continuous new strand (with the upper DNA Polymerase) → Leading Strand.
- The new strand with short fragments (Okazaki Fragments, near the lower DNA Polymerase) → Lagging Strand (and the fragments are Okazaki Fragments).
- The enzyme laying primers → DNA Primase (not yet labeled, likely a dashed box).
- The enzyme relieving supercoiling (if present, but here Helicase is unwinding, and Topoisomerase might be implied, but from the cards, focus on given labels).
For example, if we take the dashed box near the “name of strand” (lower left, with the new strand having fragments):
- The new strand with fragments is the Lagging Strand, and the fragments are Okazaki Fragments. The other new strand (continuous) is the Leading Strand. The original double - helix is the Original Strand. The single - stranded parts have SSB Protein. The small primers are from DNA Primase.
If we pick one label (e.g., the dashed box for the strand type of the lower new strand):
The lower new DNA strand (with short fragments) is synthesized discontinuously, so it’s the Lagging Strand, and the fragments are Okazaki Fragments. The upper new strand (continuous) is the Leading Strand. The parental DNA is the Original Strand. Single - stranded regions use SSB Protein. Primers are from DNA Primase.
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(Example for a dashed box labeling)
- Dashed box (lower new strand with fragments) → Lagging Strand
- Dashed box (upper new strand, continuous) → Leading Strand
- Dashed box (parental double - helix) → Original Strand
- Dashed box (single - stranded DNA) → SSB Protein
- Dashed box (primer - like) → RNA Primer
- Dashed box (enzyme for primers) → DNA Primase
- The short fragments on Lagging Strand → Okazaki Fragment
(Adjust based on the specific dashed box in the diagram; the main idea is matching each component to its function in DNA replication.)