Question

drag the steps of gene cloning into the correct sequence. ligate pastes the insulin gene into the bacterial plasmid. the recombinant plasmid containing the insulin gene is taken up by a bacterium. human insulin is isolated from the transgenic bacteria. the bacterium multiplies, producing many bacterial cells that make the insulin protein. cut out the insulin gene using restriction enzymes. cut the bacterial plasmid with the same restriction enzymes.

Explanation:

First, cut the insulin gene and plasmid with restriction enzymes. Then ligate the gene into the plasmid. Next, a bacterium takes up the recombinant plasmid. After that, the bacterium multiplies. Finally, human insulin is isolated from the transgenic bacteria.

Answer:

1. Cut out the insulin gene using restriction enzymes. Cut the bacterial plasmid with the same restriction enzymes.
2. Ligase pastes the insulin gene into the bacterial plasmid.
3. The recombinant plasmid containing the insulin gene is taken up by a bacterium.
4. The bacterium multiplies, producing many bacterial cells that make the insulin protein.
5. Human insulin is isolated from the transgenic bacteria.