Question

to kill contaminating bacteria
to allow a cell to take in plasmid dna
to alter dna sequence
to allow bacteria to grow

question 5
which list best describes the order of steps needed to clone a gene in bacteria
○ ligation, re digest, transformation
○ re digest, ligation, transformation
○ re digest, transformation, ligation
○ transformation, re digest, ligation
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Explanation:

1. RE digest: Restriction enzymes (RE) first cut the target gene and plasmid DNA to create compatible sticky ends.
2. Ligation: DNA ligase joins the cut target gene and plasmid together to form a recombinant plasmid.
3. Transformation: The recombinant plasmid is introduced into bacterial cells, which then replicate the plasmid and the cloned gene.

Answer:

RE digest, Ligation, Transformation