Question

multiple choice question
in which process are specimens plunged into an extremely cold liquid?

confocal microscopy
immunofluorescence microscopy
cryo - electron microscopy
scanning probe microscopy
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Explanation:

Cryo-electron microscopy (cryo-EM) involves rapidly plunging specimens into an extremely cold liquid (typically liquid ethane at ~-196°C) to vitrify the sample, preserving its native structure. The other microscopy techniques listed do not use this ultra-cold plunge step: confocal and immunofluorescence microscopy use fluorescent labeling for imaging, while scanning probe microscopy uses a physical probe to scan sample surfaces.

Answer:

Cryo-electron microscopy