Question

name
mutations worksheet
directions: using base pairing rules, transcribe the mrna from the dna strand. then using a codon chart, determine the amino acids (writing the amino acid abbreviations is fine). there is a codon on the last page, and in canvas.
original dna sequence: t a c a c c t t g g c g a c g a c t
original mrna sequence: aug-uag-aac-cgc-ugc-uga
(note: it makes it easier to mark the codons on the mrna with brackets)
original amino acid sequence met-stop-asn-arg-cys-stop
complete the boxes below. when determining the mutation, state whether it is a point mutation or a frameshift mutation. then, state the type for each. for a point mutation it will either be silent, missense, or nonsense. for a frameshift mutation it will either be insertion or deletion. (ex. your answer should read something like, \point mutation- missense.\)
for each of the following mutated sequences, compare them to the original dna sequence. do not compare the mutated sequences to each other
original dna sequence: t a c a c c t t g g c g a c g a c t
mutated dna sequence #1 t a c a t c t t g g c g a c g a c t
mrna mutated sequence #1: aug-uag-aac-cgc-ugc-uga

• circle the change that occurred in the mutated sequence.

(note: it makes it easier to mark the codons on the mrna with brackets)
mutated amino acid sequence met-stop-asn-arg-cys-stop
mutation type nonsense

of different amino acids (compared to original amino acid sequence) 0

Explanation:

To determine the number of different amino acids between the original and mutated sequences:

Step 1: List the original and mutated amino acid sequences

• Original: Met - Stop - Asn - Arg - Cys - Stop
• Mutated: Met - Stop - Asn - Arg - Cys - Stop

Step 2: Compare each position

1. Position 1: Met (original) vs. Met (mutated) → same.
2. Position 2: Stop (original) vs. Stop (mutated) → same.
3. Position 3: Asn (original) vs. Asn (mutated) → same.
4. Position 4: Arg (original) vs. Arg (mutated) → same.
5. Position 5: Cys (original) vs. Cys (mutated) → same.
6. Position 6: Stop (original) vs. Stop (mutated) → same.

Step 3: Count differences

Since all corresponding amino acids are identical, the number of different amino acids is \( 0 \).

Answer:

\( 0 \)