Question

part a: getting familiar with the microscope

1. notice the eyepiece, what is the magnifying power of the eye piece? ____________
2. notice the low - power objective lens: it reads “4x lens.” it is used to view the whole slide. if total magnifying power of this lens is determined by multiplying the magnifying power of the eyepiece by the magnifying power of the objective, what is the total magnifying power of this objective? ____________
3. notice the high - power objective lens: it reads “holds 40x lens.” used to view object in even greater detail. what is the total magnifying power of this lens? ____________

part b: focusing the letter “e”

1. lower the stage using the coarse adjustment knob.
2. place the slide of the letter “e” on the stage and stabilize it with the stage clips. center the “e” the best you can on the stage. the letter “e” should be facing you right side up.
3. place the lowest (i.e. 4x objective lens) into position.
4. looking into the eyepiece. slowly use the coarse adjustment to raise the stage closer to the objective lens until the object (i.e. “e”) comes into focus.
5. once the object is seen, it may be necessary to adjust the amount of light. to increase or decrease contrast, rotate the iris diaphragm slightly.
6. use the fine adjustment knob to sharpen focus if necessary.
7. in the space provided, draw an outline of the letter “e” as it appears through the eyepiece.

magnification: __4x__
total magnification: ____________

1. change magnification. place the 10x (medium power) objective lens into position. follow procedures 6 - 8. draw the outline of the letter “e” as it appears through the eyepiece.

magnification: __10x__
total magnification:____________

1. lower the stage. place the 40x objective lens into position. follow procedures 6 - 8. draw the letter “e” as it appears through the eyepiece.

magnification: __400x__
specimen: ____________
discussion question: (full sentences)

1. was the image of “e” “right side up” or “upside down”?
2. what happens to the microscope image when you move the microscope slide towards you?
3. what happens to the image when you move the microscope slide away from you?

Explanation:

Step1: Determine eyepiece magnification

Typically, the eyepiece magnification of a light - microscope is 10X.

Step2: Calculate total magnification for 4X objective

Total magnification = Eyepiece magnification×Objective magnification. So, for a 4X objective, Total magnification = 10×4 = 40X.

Step3: Calculate total magnification for 40X objective

Using the same formula, for a 40X objective, Total magnification = 10×40 = 400X.

Step4: Answer discussion question 1

Microscopes invert the image. So the image of “e” is upside - down.

Step5: Answer discussion question 2

When you move the microscope slide towards you, the image moves away from you in the microscope view.

Step6: Answer discussion question 3

When you move the microscope slide away from you, the image moves towards you in the microscope view.

Answer:

1. 10X
2. 40X
3. 400X

Discussion Question:

1. The image of “e” is upside - down.
2. The image moves away from you.
3. The image moves towards you.