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Question
question 3
it is important that you understand the steps in the extraction procedure and why each step was necessary. each step in the procedure aided in isolating the dna from other cellular materials. match the procedure with its function:
prompts answers
1 filter dna mixture select match
2 add salt and soap to dna mixture select match
3 blending dna source in the blender select match
4 addition of alcohol to filtered extract select match
Brief Explanations
- Filtering the DNA mixture removes large - sized cellular debris.
- Adding salt and soap to the DNA mixture helps to break open cells (lyse cells) and clump cellular proteins, separating them from DNA.
- Blending the DNA source in the blender physically breaks down the tissue to release cells and DNA.
- Adding alcohol to the filtered extract causes DNA to precipitate out of the solution as DNA is insoluble in alcohol.
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- Removes large - sized cellular debris
- Lyses cells and clumps proteins
- Physically breaks down tissue
- Causes DNA to precipitate