Question

rna primase, ligase, helicase, polymerase, topoisomerase
unwinds the dna molecule
synthesizes the complementary daughter strands
synthesizes rna primer at the initiation point
creates nicks ahead of the replication fork to relieve tension from unwinding
joins the okazaki fragments together

Explanation:

Each enzyme has a specific, well-defined role in DNA replication:

1. Helicase breaks hydrogen bonds to unwind the double-stranded DNA molecule.
2. DNA polymerase adds complementary nucleotides to synthesize new daughter DNA strands.
3. RNA primase synthesizes a short RNA primer that acts as the starting point for DNA polymerase.
4. Topoisomerase creates small nicks in the DNA ahead of the replication fork to release the torsional tension caused by unwinding.
5. DNA ligase seals the gaps between the short Okazaki fragments on the lagging strand to form a continuous DNA strand.

Answer:

• unwinds the DNA molecule → helicase
• synthesizes the complementary daughter strands → polymerase
• synthesizes RNA primer at the initiation point → RNA primase
• creates nicks ahead of the replication fork to relieve tension from unwinding → topoisomerase
• joins the Okazaki fragments together → ligase