Question

we need to prepare a master mix with the reagents for each reaction. what is included in the master mix?
a) ethanol, nad⁺, and buffer
b) adh, nad⁺, and ethanol
c) ethanol, nadh, and buffer
d) adh, nad⁺, and nadh

Explanation:

This refers to an alcohol dehydrogenase (ADH) reaction master mix. ADH is the enzyme catalyst, NAD⁺ is the cofactor that accepts electrons, and ethanol is the substrate for the reaction. These are the core shared reagents for replicate reactions in this assay.

Answer:

b) ADH, NAD⁺, and Ethanol