Question

what will happen if you dont heat - fix?
○ aerosol will be generated
○ cells will wash away
○ cell morphology will be changed
○ more stain will penetrate

Explanation:

Heat - fixing is a process in microbiology (related to Biology, a subfield of Natural Science) that adheres bacterial cells to the slide. If we don't heat - fix, when we perform subsequent washing steps (like during staining procedures), the cells are not attached firmly to the slide and will be washed away. Generating aerosol is more related to improper handling like vigorous shaking or incorrect pipetting rather than not heat - fixing. Changing cell morphology is more associated with improper heat - fixing (like over - heating) rather than not doing it at all. The amount of stain penetration is not directly related to the absence of heat - fixing.

Answer:

B. cells will wash away