Question

what are the two things that need to be done to a spectrometer prior to properly using it
set the correct absorbance and prime the light emitter
set the correct wavelength and set the correct absorbance
zero to a blank sample and set the correct absorbance
set the correct wavelength and zero to a blank sample
what is the order of numbers (top to bottom) to set a p1000 to 850 ul?
8 - 5 - 0
0 - 8 - 5
5 - 8 - 0
0 - 5 - 8
what is the order of numbers (top to bottom) to set a p200 to 20 ul?
0 - 2 - 0
2 - 0 - 0
0 - 0 - 2
2 - 2 - 2

Explanation:

1. For a spectrometer, setting the correct wavelength is crucial as different substances absorb light at specific wavelengths, and zero - ing to a blank sample helps to account for any background absorbance.
2. A P1000 pipette has a range of 100 - 1000 μl. To set it to 850 μl, the numbers from top to bottom are 8 - 5 - 0.
3. A P200 pipette has a range of 20 - 200 μl. To set it to 20 μl, the numbers from top to bottom are 0 - 2 - 0.

Answer:

1. Set the correct wavelength and zero to a blank sample
2. 8-5-0
3. 0-2-0