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Question
which of the following statements is not true for dapi staining? a) dapi stains the cell nuclei b) the light source only emits uv light c) dapi emits blue light d) dapi is excited by uv light
DAPI (4',6 - diamidino - 2 - phenylindole) is a fluorescent dye that binds strongly to DNA and is used to stain cell nuclei. It is excited by UV light and emits blue light. However, the light source used for DAPI staining is not limited to only emitting UV light; it can also have other wavelengths for various experimental set - ups and considerations.
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B. The light source only emits UV light