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Question
- while performing a pipetting exercise, you mix 100 μl yellow dye and 25 μl red dye in a tube. a. what is the final volume of solution in the tube in μl? b. what are the volume proportions of the two colors in the final tube?
Step1: Calculate final volume
Add the volumes of the two dyes. $V = 100+25=125\ \mu L$.
Step2: Calculate volume proportion of yellow dye
The proportion of yellow dye is $\frac{100}{125}=\frac{4}{5}$.
Step3: Calculate volume proportion of red dye
The proportion of red dye is $\frac{25}{125}=\frac{1}{5}$.
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a. $125\ \mu L$
b. Yellow dye: $\frac{4}{5}$, Red dye: $\frac{1}{5}$