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based upon fig. 5, guichard et al (2013) showed that the actin used in the cytoskeleton was not connected to tight junctions in cholera infected cells. more concentrated in cholera infected cells compared to healthy cells. was more evenly distributed in cholera infected cells compared to healthy cells. was absent from the membranes in healthy cells, but present in cholera infected cells. not present in cholera infected cells.
Without seeing Fig. 5, we can only analyze based on general knowledge of cell - biology related to cholera. Actin is a key component of the cytoskeleton. In cholera - infected cells, changes in the cytoskeleton can occur. Typically, in such infections, the normal organization of the cytoskeleton is disrupted. Actin is not necessarily more concentrated or evenly distributed in infected cells compared to healthy cells. It is also not absent from membranes in healthy cells or only present in infected cells. And it is known that in cholera - infected cells, the connection between actin in the cytoskeleton and tight junctions can be disrupted.
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The first option: not connected to tight junctions in Cholera infected cells.