Question

1. how do you calculate magnification on a light microscope? answer magnification = objective magnification x eyepiece magnification. 2. explain how to focus a microscope using the high - power lens. include any safety issues you need to be aware of. score for question 2: _ of 2 points answer: focus on low - power first. switch to high - power. use fine adjustment knob. keep equipment clean. avoid lens - slide contact. 3. compare your observations of micrographs obtained from the optical microscope, the scanning electron microscope, and the transmission electron microscope. score for question 3: _ of 3 points answer: optical microscopes have lower resolution, sem shows surface details, tem shows internal details. 4. which type of microscope would be the best tool to use to view the surface of a bacterial cell? why? score for question 4: _ of 3 points

Explanation:

1. Answer: Multiply the objective lens magnification by the eyepiece magnification.

• Brief Explanations: In a light - microscope, the total magnification is determined by multiplying the magnification power of the objective lens and the eyepiece lens. This is a fundamental principle in microscopy for obtaining the overall enlarged view of the specimen.

1. Answer: First, focus on low - power. Switch to high - power. Use fine adjustment knob. Keep equipment clean. Avoid lens - slide contact.

• Brief Explanations: When focusing a microscope with a high - power lens, start with low - power to locate the specimen. Then switch to high - power and use the fine adjustment for sharp focus. Keeping the equipment clean and avoiding lens - slide contact are safety and maintenance measures.

1. Answer: Optical microscopes have lower resolution compared to transmission electron microscopes. Scanning electron microscopes show surface details while transmission electron microscopes show internal details.

• Brief Explanations: Optical microscopes use light and have limitations in resolution. Electron microscopes use electrons and can achieve higher resolution. SEM and TEM have different imaging capabilities based on their design for surface and internal structure imaging respectively.

1. Answer: Scanning Electron Microscope (SEM).

• Brief Explanations: SEM is best for viewing surface details of a specimen like the surface of a bacterial cell as it provides a 3 - D like view of the surface features.

Answer:

1. Answer: Multiply the objective lens magnification by the eyepiece magnification.

• Brief Explanations: In a light - microscope, the total magnification is determined by multiplying the magnification power of the objective lens and the eyepiece lens. This is a fundamental principle in microscopy for obtaining the overall enlarged view of the specimen.

1. Answer: First, focus on low - power. Switch to high - power. Use fine adjustment knob. Keep equipment clean. Avoid lens - slide contact.

• Brief Explanations: When focusing a microscope with a high - power lens, start with low - power to locate the specimen. Then switch to high - power and use the fine adjustment for sharp focus. Keeping the equipment clean and avoiding lens - slide contact are safety and maintenance measures.

1. Answer: Optical microscopes have lower resolution compared to transmission electron microscopes. Scanning electron microscopes show surface details while transmission electron microscopes show internal details.

• Brief Explanations: Optical microscopes use light and have limitations in resolution. Electron microscopes use electrons and can achieve higher resolution. SEM and TEM have different imaging capabilities based on their design for surface and internal structure imaging respectively.

1. Answer: Scanning Electron Microscope (SEM).

• Brief Explanations: SEM is best for viewing surface details of a specimen like the surface of a bacterial cell as it provides a 3 - D like view of the surface features.