Question

1. locate the diaphragm under the stage. move it and record the changes in light intensity as you do so.

part iv, concluding questions:

1. discuss 3 procedures which should be used to properly handle a light microscope.
2. explain why the light microscope is also called the compound microscope.
3. images observed under the light microscope are reversed and inverted. explain what this means.
4. explain why the specimen must be centered in the field of view on low power before going to high power.
5. a microscope has a 20x ocular (eyepiece) and two objectives of 10x and 43x respectively.
6. calculate the low power magnification of this microscope. show your formula and all work.

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Explanation:

Step1: Recall microscope - handling procedures

1. Carry with two hands (one on arm, one under base).
2. Keep it clean with lens - paper.
3. Store properly covered.

Step2: Explain compound - microscope name

It has two lens systems (ocular and objective), so called compound.

Step3: Define reversed and inverted image

Reversed means left - right reversed, inverted means up - down inverted.

Step4: Reason for centering specimen

High - power has a smaller field of view. Centering on low - power ensures specimen stays in view on high - power.

Step5: Recall magnification formula

Magnification = Ocular magnification × Objective magnification.

Step6: Identify low - power objective

The lower power objective is 10X.

Step7: Calculate low - power magnification

Given ocular magnification = 20X, objective magnification = 10X. Magnification = 20×10 = 200X.

Answer:

1. 1. Carry with two hands (one on arm, one under base). 2. Keep it clean with lens - paper. 3. Store properly covered.
2. It has two lens systems (ocular and objective), so called compound.
3. Reversed means left - right reversed, inverted means up - down inverted.
4. High - power has a smaller field of view. Centering on low - power ensures specimen stays in view on high - power.
5. Magnification formula is Magnification = Ocular magnification × Objective magnification.
6. 200X