Question

manuel’s teacher asked the class to make a wet mount slide of their cheek cells. manuel made his slide and placed it on the microscope stage. then he got ready to view his cheek cells by following these procedures.

1. looking through the eyepiece, manuel adjusted the diaphragm so field of vision was lit.
2. manuel looked at the stage from the side and lowered the body tube so the high power objective was almost touching the slide.
3. looking into the eyepiece, he very slowly turned the coarse adjustment to bring the cells into focus.

what suggestion would you make to manuel to correctly use the microscope to view his cheek cells?
a close the diaphragm
b start with the low power objective
c do not look at the stage when lowering the body tube
d start with the body tube raised and lower it while looking through the eye piece

Explanation:

To view cheek cells, the correct microscope procedure starts with the low - power objective. The low - power objective has a larger field of view and longer working distance, which makes it easier to locate the specimen first. Manuel started with the high - power objective, which is incorrect. Option A is wrong because the diaphragm should be adjusted to let in appropriate light, not closed. Option C is wrong as looking at the stage when lowering the body tube (to prevent the objective from hitting the slide) is correct. Option D is wrong as the proper way is to lower the body tube while looking at the stage (not through the eyepiece) when using the coarse adjustment initially.

Answer:

B. start with the low power objective