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match negative staining and shadowing in tem with the types of specimens that are visualized.
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negative staining
shadowing
used to visualize viruses and cellular microbes
used to study bacterial flagella and dna
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Negative staining in TEM uses an acidic stain that does not penetrate the specimen, creating a dark background around light, intact microbes (including viruses and cellular microbes) for visualization. Shadowing involves coating a specimen with a heavy metal at an angle, which creates a shadow effect ideal for highlighting the delicate, thin structures like bacterial flagella and DNA strands.
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Negative staining → Used to visualize viruses and cellular microbes
Shadowing → Used to study bacterial flagella and DNA